Tau Thr181 detection

Tau Thr181 Detection

Detection of tau protein phosphorylated at threonine 181 (Tau Thr181) is pivotal in Alzheimer’s disease research and diagnosis. One common method is the enzyme-linked immunosorbent assay (ELISA), which utilizes specific antibodies to quantify Tau Thr181 levels in biological samples, offering high sensitivity and specificity.

Another technique is the Homogeneous Time-Resolved Fluorescence (HTRF) assay, which employs fluorescence resonance energy transfer between donor and acceptor molecules to detect Tau Thr181, enabling rapid and precise measurements in a homogeneous format.

Additionally, mass spectrometry provides a powerful approach for simultaneously quantifying multiple tau species, including Tau Thr181, allowing for detailed analysis of tau isoforms and post-translational modifications in complex biological samples.