ATP Detection Kit

ATP Detection Kit

When selecting an ATP detection kit for environmental and biomedical applications, the Tribioscience ATP Colorimetric/Fluorometric Assay Kit (Catalog #TBS2010) stands out for its sensitivity and versatility. This kit employs the phosphorylation of glycerol to produce a measurable product, detectable via colorimetric (OD = 570 nm) or fluorometric (Ex/Em = 535/590 nm) methods. It can detect ATP concentrations as low as 0.5 µM in various samples, making it suitable for direct assays in cells and other biological materials, as well as for assessing enzymes that produce or degrade ATP.

The kit’s design emphasizes simplicity and efficiency, requiring only 10 µL samples and delivering results in under 30 minutes. Its detection range spans from 0.5 to 1000 µM in a 96-well plate format, facilitating high-throughput analyses. This makes it particularly advantageous for laboratories needing rapid and reliable ATP quantification across multiple samples.

For optimal performance, the kit should be stored at -20°C, with a shelf life of three months. Prior to use, all components except the enzyme should be brought to room temperature, and small vials should be briefly centrifuged before opening. These handling procedures ensure the kit’s robustness and reliability, making it a valuable tool for researchers in both environmental monitoring and biomedical fields.