Tth DNA Polymerase (TBP0161)

Description

Tth DNA Polymerase is a thermostable enzyme from the eubacterium Thermus thermophilus strain HB8 that works best at 74 °C and can last about 20 minutes at 95 °C. It builds DNA strands in the 5´→3´ direction using DNA templates (with magnesium) or RNA templates (with manganese). It is commonly used in high-temperature applications like PCR, RT-PCR, reverse transcription, and primer extension.

Specification

Concentration: 5u/µl

Storage buffer: 10mM Tris-HCl, 1mM dithiothreitol, 0.1 mM EDTA, 300mM KCl, 0.1% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.5 (25°C).

Reaction Buffer:

1. 5× RT/PCR reaction buffer (One Step-buffer): 250 mM bicine (pH 8.2, by KOH, at 25 °C), 580mM KOAC, 40% Glycerol
2. 10× PCR buffer: 100 mM Tris-HCl, (pH 8.8 at 25 °C), 15 mM MgSO4, 800mM (NH4)2SO4, 0.5 mg/ml BSA, 0.5% Tween 20

Application

PCR and RT-PCR- cDNA synthesis

Research use only.

Additional information

Datasheet:

TBP0161-Tth DNA Polymerase_datasheet

MSDS:

TBP0161_Tth DNA Polymerase_MSDS

Research use only.