Protocatechuate 3,4-dioxygenase, Enzyme Activity (TBP0036)

$293.00$468.00

SKU: TBP0036 Category:
SKUStock SIZE PriceQuantity
TBP0036-25U Yes 25 U $293.00
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TBP0036-50U Yes 50 U $468.00
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Description

Preparation and Specification

Appearance: Light brown amorphous powder, lyophilized

Activity: GradeⅢ 3.0U/mg-solid or more

Contaminant: NADPH oxidase ≤1.0×10⁻¹%

Stabilizers: Sugars

 

Properties

Stability: Stable at -20°C

Molecular weight: approx. 600,000 (by gel filtration)

Michaelis constant: 1.85×10⁻⁵M (Protocatechuate)

Structure: Protein with nonheme iron

Inhibitors: Ag⁺, Hg⁺⁺

Optimum pH: 9.0

Optimum temperature: 60-65°C

pH Stability: pH 6.0-9.5 (25°C, 72hr)

Thermal stability: below 55°C (pH 7.5, 1hr)

 

Applications

This enzyme is useful for enzymatic determination of choline esterase when coupled with p-hydroxybenzoate hydroxylase (HBH-311).

Additional information

SIZE

50 U, 25 U

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