Hexokinase, Enzyme Activity (TBP0029)

Description

Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 150U/mg-solid or more

Contaminants: Phosphoglucose isomerase ≤1.0×10⁻¹%

6-Phosphogluconate dehydrogenase ≤1.0×10⁻²%

Glucose-6-phosphate dehydrogenase ≤1.0×10⁻²%

Myokinase ≤1.0×10⁻²%

Glutathione reductase ≤ 5.0×10⁻¹%

 

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 82,000 (by gel filtration)

Isoelectric point: 4.1±0.1

Michaelis constants: 2.3×10⁻⁴M (D-Glucose), 7.7×10⁻⁵M (ATP)

Inhibitors: Metal ions, p-chloromercuribenzoate, iodoacetamide, SDS, etc

Optimum pH: 8.0-9.0

Optimum temperature: 50°C

pH Stability: pH 4.0-9.0 (25°C, 20hr)

Thermal stability: below 45°C (pH 7.0, 30min)

 

Applications

The enzyme is useful for enzymatic determination of glucose, adenosine-5′-triphosphate (ATP) and creatine phosphokinase when coupled with glucose-6-phosphate dehydrogenase (=G-6-PDH, G6D- 311, G6D-321).

 

Additional information

SIZE	1000 U, 5000 U

Datasheet:

TBP0029-Hexokinase

MSDS:

TBP0029_Hexokinase Safety Data Sheet