Glutamate dehydrogenase(NADP dependent), Enzyme Activity (TBP0023)
$160.00 – $640.00
SKU | Stock | SIZE | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0023-1KU | Yes | 1000 U | $160.00 | |||
TBP0023-5KU | Yes | 5000 U | $640.00 |
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Description
Preparation and Specification
Appearance: Solution with 50mM Tris-HCl buffer containing 0.05% NaN₃ and 5.0mM EDTA, pH 7.8
Activity: GradeⅡ∙Ⅲ 300U/mg-protein or more (9,000U/ml or more)
Contaminants: NADPH oxidase ≤1.0×10⁻²%
Glutathione reductase ≤1.0×10⁻²% (GradeⅡ-209) ≤1.0×10⁻¹% (GradeⅢ-309)
Stabilizer: Ethylenediaminetetraacetic acid (EDTA)
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 300,000
Isoelectric point: 4.6
Michaelis constants: 1.1×10⁻³M (NH₃), 3.4×10⁻⁴M (α-Ketoglutarate)
1.2×10⁻³M (L-Glutamate), 1.4×10⁻⁵M (NADPH), 1.5×10⁻⁵M (NADP⁺)
Structure: 6 subunits (M.W.50,000) per enzyme molecule
Inhibitors: Hg⁺⁺, Cd⁺⁺, p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine, 2,2′-dithiopyridine
Optimum pH: 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG)
Optimum temperature: 45°C (α-KG→L-Glu) 45-55°C (L-Glu→α-KG)
pH Stability: pH 6.0-8.5 (25°C, 20hr)
Thermal stability: below 50°C (pH 7.4, 10min)
Applications
This enzyme is useful for enzymatic determination of NH₃, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis.
Additional information
SIZE | 1000 U, 5000 U |
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Datasheet:
TBP0023-Glutamate dehydrogenase(NADP dependent)
MSDS:
TBP0023_Glutamate dehydrogenase(NADP dependent) Safety Data Sheet
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