Tribo™ DNase I Solution (1 mg/mL)

Tribo™ DNase I Solution (1 mg/mL)

$89.00

DNase I (RNase-Free) is an endonuclease that efficiently hydrolyzes double- (ds) or single stranded DNA to a mixture of a short oligo- and mononucleotides.

SKU: TBS6022 Category:
SKUStock SIZE (mg) PriceQuantity
TBS6022Yes1 mg $89.00
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Description

DNase I (RNase-Free) is an endonuclease that efficiently hydrolyzes double- (ds) or single stranded DNA to a mixture of a short oligo- and mononucleotides. In the presence of Mg2+, cleavage of each strand of a dsDNA substrate proceeds independently. In contrast in the presence of Mn2+, the enzyme cleaves both strands of DNA at approximately the same site to generate molcuels with blunt ends or 1- or 2-base overhangs that can be blunted with T4 DNA Polymerase.
The DNase I Solution is provided at a concentration of 1 mg/mL (2000U/mL). 10x Reaction Buffer and Stop Solution are included in the kit.

Applications:

  • Elimination of the DNA template following in vitro RNA synthesis with T7 Phage RNA Polymerase.
  • Characterization of DNA: protein interactions by one of “DNase I footprinting”.
  • Treatment of RNA prior to RT-PCR.
  • Radiolabeling of DNA by nick translation.

Kit Contents:

CompositionConcentrationVolume
DNase I1 mg/mL1.0mL
Reaction Buffer10x1.5mL
Stop Solution10x1.5mL

DNase I: 1mg=2000U

Storage Conditions:

The kit is shipped on blue ice. Keep all components at -20℃ for long-term storage.
Shelf life of 12 months after receiving.

Additional information

SIZE (mg)

1 mg

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