Tribo™ Human IFN-Gamma ELISA Kit (TBS3230)
$390.00
The Tribo™ Fast Human IFN-γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma.
SKU | Stock | SIZE | Price | Quantity | ||
---|---|---|---|---|---|---|
TBS3230 | Yes | 1 x 96-well plate | $390.00 |
- Description
- Terms
- Additional information
- Documents
- References
- Reviews (1)
Description
The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour instead of 5-6 hours (Fig. 1). The detection range is from 20 to 15000 pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.
Sensitivity
The minimum detectable dose (MOD) of human IFN-γ is typically 8 pg/ml.
Specificity
This assay recognizes natural and recombinant human IFN- γ.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Contents & Storage Conditions
Part | Part # | Description | Storage of Opened/Reconstituted |
---|---|---|---|
Human IFN-γ Microplate | TBS3230A | 96-well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN- γ. | Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
Human IFN-γ Standard | TBS3230B | 100 µL of Recombinant human IFN- γ protein (145.8ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS3230C | 2.2 ml of HRP-Human IFN- γ antibody. | May be stored for up to 3 months at 2-8℃ |
Assay Diluent | TBS3230D | 12 ml of a buffered protein base with preservatives. | |
Wash Buffer | TBS3000W | 12 ml of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12 ml of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6 ml of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Additional information
SIZE | 1 x 96-well plate |
---|
Ao, Z., Wu, Z., Cai, H., Hu, L., Li, X., Kaurich, C., Chang, J., Gu, M., Cheng, L., Lu, X., & Guo, F. (2022). Rapid Profiling of Tumor-Immune Interaction Using Acoustically Assembled Patient-Derived Cell Clusters. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(22), e2201478. https://doi.org/10.1002/advs.202201478
1 review for Tribo™ Human IFN-Gamma ELISA Kit (TBS3230)
Add a review
You must be logged in to post a review.
HELEN W –
sample tested: cell culture supernant, human primary NK cells
quick, easy to follow protocol