Human Total GAPDH ELISA (TBS32102)

Description

GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase) is an enzyme that is involved in the second phase of glycolysis, a process that breaks down glucose and ultimately produces pyruvate for the trichloroacetic acid cycle (TCA cycle), and adenosine triphosphate (ATP), which is the primary energy source for important biological functions.

GAPDH is generally thought to be present at a constant level within the cell. In most cases, this stays true regardless of experimental treatment or technical procedures. Because of its ability to remain present, the measurement of GAPDH is used as an internal control for experimental error. GAPDH is widely used in different assay methods to investigate certain aspects of cell activity.

The Human Total GAPDH ELISA Kit is used to measure the presence of human GAPDH in cell lysates.

 

Typical Data

This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.

Sensitivity

The limit of detectable dose (LOD) of GAPDH is 0.15625ng/ml.

Specificity

This assay measures the total human/mouse/rat GAPDH in cell lysates.

 

Simple Procedures

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human Total GAPDH Microplate	TBS32102A	96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human GAPDH.	The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human GAPDH Standard	TBS32102B	100 μL of Recombinant human GAPDH protein (200 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS32102C	12 mL of human biotinylated GAPDH antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS32102D	12 mL of Streptavidin-HRP
Assay Diluent	TBS32102E	12 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on at least two 96-well plates.

 

Related Product

1. Human IL-1ß ELISA (TBS3219)
2. Human IL-2 ELISA (TBS3220)
3. Human IL-4 ELISA (TBS3221)
4. Human IL-6 ELISA (TBS3223)
5. Human IL-7 ELISA (TBS3224)

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS32102_Total_human_GAPDH_ELISA_V01_2022

MSDS:

TBS32102_Human_GAPDH_ELISA_MSDS