Human Menin ELISA (TBS32100)

$810.00

PBMC, Cell and Tissue Menin

SKU: TBS32100 Category:
SKUStock SIZE PriceQuantity
TBS32100 Yes 1 x 96-well plate $810.00
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Description

Human Menin ELISA is a solid phase ELISA designed to measure human Menin levels in cell culture supernatants, serum, plasma and cell lysates. The detection range is from 0.18 to 150 ng/mL. The levels of human Menin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human Menin protein.

Typical Data

This standard curve(R2=1.000) is provided in Fig. 3 for demonstration only. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human Menin is typically 100 pg/ml.

The Intra-CV is < 10%, the Inter-CV is < 10%.

Specificity

This assay recognizes natural and recombinant human Menin.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

Accurate Standard Curve

 

Kit Content and Storage Conditions

PART PART# DESCRIPTION STORAGE OF OPENED/ RECONSTITUTED
Human Menin Microplate TBS32100A 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human Menin. The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human Menin Standard TBS32100B 15 μL of Recombinant human Menin protein (10 μg/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS32100C 20 μL of human biotinylated Menin antibody (600x).  

May be stored for up to

3 months at 2-8 °C.

Detection B TBS32100D 40 μL of Streptavidin-HRP (300x)
Assay Diluent TBS32100E 25 mL of a buffered protein base with preservatives.
10x Wash Buffer TBS3000W 50 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Research use only.

Additional information

SIZE

1 x 96-well plate

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