Human Menin ELISA (TBS32100)

Description

Human Menin ELISA is a solid phase ELISA designed to measure human Menin levels in cell culture supernatants, serum, plasma, and cell lysates. The main feature is that the kit makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, not 5-6 hours (Fig. 1), and only includes one washing step before color development. The detection range is from 1.638 to 400 ng/mL. The levels of human Menin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human Menin protein.

 

Typical Data

This standard curve(R²=1.000) is provided in Fig. 3 for demonstration only. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human Menin is typically 0.6 ng/ml.

The Intra-CV is < 10%, and the Inter-CV is < 10%.

Specificity

This assay recognizes natural and recombinant human Menin.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human Menin Microplate	TBS32100A	96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human Menin.	The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human Menin Standard	TBS32100B	60 μL of Recombinant human Menin protein (20ug/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS32100C	12 mL of human biotinylated Menin antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS32100D	12 mL of Streptavidin-HRP
Assay Diluent	TBS32100E	12 mL of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Product

1. Human IL-1ß ELISA (TBS3219)
2. Human IL-2 ELISA (TBS3220)
3. Human IL-4 ELISA (TBS3221)
4. Human IL-6 ELISA (TBS3223)
5. Human IL-7 ELISA (TBS3224)

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS32100_human_menin_elisa_V01_2022

MSDS:

TBS32100_Human_Menin_ELISA_MSDS