Lactobacillus Reuteri qPCR Kit (TBS42058)

$339.00$678.00

Alternative names: Lactobacillus reuteri; L. reuteri

SKU: TBS42058 Categories: ,
SKUStock SIZE PriceQuantity
TBS42058-100 Yes 100 Reactions $339.00
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TBS42058-200 Yes 200 Reactions $678.00
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Description

Lactobacillus reuteri is a species of gram-positive, rod-shaped probiotic bacterium that naturally lives in the digestive tract of humans and animals. Its detection is critical for probiotic research, microbiome studies and product quality control. Tribioscience Lactobacillus reuteri qPCR Detection Kit is specifically used to detect Lactobacillus reuteri species in a single real-time quantitative PCR (qPCR) reaction using fluorescent probes. Detection of the target DNA confirms ingredient authenticity and helps prevent food fraud.

PRINCIPLE

The kit is based on probe real-time qPCR technology, which amplifies specific gene sequence of Lactobacillus reuteri. The target-specific fluorescent probe bind to the amplified PCR products, and as the products accumulate, the fluorescence signal is increased in PCR amplification. By monitoring fluorescence intensity in real time, the kit allows quantitative detection of the target DNA during PCR amplification, providing rapid and accurate results.

KEY FEATURES

• High sensitivity and specificity for Lactobacillus reuteri.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: just add DNA Template and water.
• No cross reactivity with other species.

APPLICATIONS

qPCR detection of Lactobacillus reuteri is used in food and environmental, fermentation process control, spoilage prevention, and microbial ecology studies to rapidly and specifically quantify the bacteria.

KIT CONTENTS

Name 100x rxn 200x rxn
qPCR Super Mix (B1) 0.9mL 1.8mL
Primer-probe Mix (B2) 0.5mL 1mL
Positive Control DNA (B+) 10µL 20µL
Negative Control DNA (B) 60µL 120µL

The Lactobacillus reuteri target probe has been labeled with FAM while the PCR internal control has been labeled with Hex.

STORAGE CONDITION

The kit is shipped on ice and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

DNA Standard PCR amplification

DNA Standard Curve

Research use only.

Additional information

SIZE

200 Reactions, 100 Reactions

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