Maize GMO CBH-351 qPCR Detection

$459.00

SKU: TBS43002 Categories: ,
SKUStock SIZE PriceQuantity
TBS43002-100 Yes 100 Reactions $459.00
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Description

Many countries in the world regulate the cultivation and trade with genetically modified organisms (GMO). The enforcement of such regulations depends on the ability to detect and quantify the presence of GMOs in food, feed, and seed products. The real-time qPCR method is sensitive and robust and is regarded as the gold standard for GMO analysis.

The Maize GMO CBH-351 qPCR Kit is designed for identifying GMO marker CBH-351 presence or absence in food, feed, and seed products using real-time quantitative polymerase chain reaction(qPCR), primers, and labeled probe. The Kit includes maize GMO CBH-351 Positive and Negative controls, PCR internal controls, qPCR Super Mix, maize GMO CBH-351 Prime-Probe Mix, in which the probe is labeled with Fam, and Hex is labeled for PCR internal control. These aids in the straightforward interpretation of the results.

KEY FEATURES

  • Highly sensitivity and specificity for identification of GMO CBH-351.
  • High efficiency: the optimal systemic conditions for PCR amplification.
  • Streamlined protocol: Just add DNA Template and water.
  • No cross reactivity with others.

PCR PROTOCOL

1. DNA extraction: The methods for DNA extraction can be used for any suitable preparation of DNA purification from food samples. We recommend that the Fast genomic DNA extraction method be used for this purpose (catalog: TBS6008).

2. Set up a PCR reaction for each sample in 20 µL

Reaction Component Volume (µL)
qPCR Super Mix 7.0
Primer-probe Mix 5.0
Nuclease-free Water 3.0
DNA sample 5.0
Final Volume 20 µL

Internal control should be included as below: Positive Control or Negative control (5 µL /reaction).

3. Suggested PCR conditions

Step Amplification PCR
HOLD CYCLE (40 cycles)
Denature Anneal/ Extend
Temperature 95 °C 95 °C 60 °C
Time 1 min 15 sec 60 sec

 

RELATIVE PRODUCTS

  1. TBS6008: Fast Genomic DNA Extraction
  2. TBS6025: Microbial DNA Magnetic Extraction
  3. TBS43001: Maize GMO 98140 qPCR Detection
  4. TBS43003: Maize GMO DBT418 qPCR Detection
  5. TBS43004: Maize GMO LY038 qPCR Detection

Additional information

SIZE

100 Reactions

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