Horse QPCR Kit (TBS42004)

Description

The Horse QPCR Kit is designed for the target-DNA specific detection of horse DNA in samples like food and animal feed ingredients. The assay uses a real-time PCR-based protocol with fluorescent-probe to detect target DNA. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for horse (Fig.1-2, and Table 1). No cross reactivity is observed with other animal species (See Table 2). The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues, or health concerns.

PRINCIPLE

Authenticating horse ingredients using real-time probe-qPCR is based on the amplification of a specific region of the relevant target genome. The amplified product is detected using target-specific fluorescent probes that bind to the amplified product. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run allows the detection of the accumulating PCR product in real time.

The Horse qPCR Kit includes Horse positive and negative Controls, and PCR internal controls, qPCR super mix, prime-probe mix, in which the probe is labeled with FAM for Horse DNA target, and Hex is labeled for PCR internal control. These aid in the straightforward interpretation of the results.

KEY FEATURES

❖ Highly sensitivity and specificity for Horse derived DNA.
❖ High efficiency: the optimal systemic conditions for PCR amplifications.
❖ Streamlined protocol: Just add DNA Template, and water.
❖ No cross reactivity with other species.

APPLICATIONS

Detect horse-derived DNA in food, animal feed, and other samples.

KIT CONTENTS

The Horse DNA probe is labeled with FAM, and PCR internal control is labeled with Hex.

STORAGE CONDITION

The kit is shipped on ice and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

Fig. 1: Horse DNA qPCR Amplification

Fig. 2: DNA Concentration and qPCR amplification

Research use only.

Additional information

Data Sheet:

TBS42004 Horse_qPCR kit_V02-2026

MSDS:

TBS42004_HORSE_QPCR_MSDS_2026

Research use only.