Tribo™ Bovine QPCR, 100 reactions

Description

The Bovine QPCR Kit is designed for the target-DNA specific detection of bovine matter in food and animal feed ingredients. The assay uses a real-time PCR-based protocol with fluoresce-probe to detect target DNA. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for bovine (Fig.1-2, and Table 1). No cross reactivity is observed with other animal species (See Table 2). The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues, or health concerns.

 

Key Features

• Highly sensitive and specific for bovine derived DNA.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: just add DNA template and water.
• No cross reactivity with other species

 

Storage Conditions

The kit is shipped on ice, and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

 

PCR Protocol

1. Set up PCR reaction for each sample in 25 µL

Internal control should be included as below: Positive Control (5 µL /reaction), or Negative Control (5 µL/reaction)

2. Suggested PCR conditions

 

Fig. 1: Bovine qPCR Amplification

Fig.2: DNA Concentration and qPCR amplification

 

Table1. Bovine qPCR Sensitivity Data

 

Table2: Cross-reactivity Survey

 

Additional information

SIZE	100 Reactions

Data Sheet:

TBS42001_Bovine_qPCR kit_V03_2022

MSDS:

TBS42001_CATTLE_QPCR_MSDS 2022