Articles

ATP Colorimetric/Fluorometric Assay Kit

Adenosine-5'-triphosphate (ATP) is a multifunctional nucleotide used in cells as a coenzyme. ATP is the main energy source for the majority of cellular functions. ATP also is critically involved in maintaining cell structure by facilitating assembly and disassembly of elements of the cytoskeleton. Tribioscience’s ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method that utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (OD = 570 nm) or fluorometric...

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HRP Fluoresce Substrate system

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also known as Amplex Red or Ampliflu Red, is not only a sensitive and stable fluorogenic substrate for horseradish peroxidase (HRP), it is an ultrasensitive probe for H2O2. In the presence of HRP and H2O2, ADHP generates a highly fluorescent resorufin that fluoresces red with maximum absorption of 570nm and maximum emission of 585nm. ADHP is currently the most sensitive and stable fluorogenic probe for detecting HRP and H2O2 and has been used widely to detect HRP in...

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Fast Genomic DNA Extraction from Cells and Tissues

Fast DNA extraction kit provides a simple and rapid method to isolate the genomic DNA from tissues or cells for PCR amplification, genotype identification, CRISPR mutation detection, and library screening. The formulated reagent is ready to use in a single reagent without other preparation. No organic extraction and alcohol precipitation is needed, and multiple samples can be easily processed simultaneously. Fast Genomic DNA Extraction from Cells and Tissues Related Products: Fast Genotyping System (Mouse, Rat, or Zebrafish) (TBS4033)Mouse Tail Fast DNA Extraction Kit...

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Caspase-3 Fluorometric Assay kit

Apoptosis plays a fundamental role in many normal biological processes as well as in several disease states. Apoptosis can be induced by various stimuli that all produce the same result: systematic and orderly cell death. Caspases cleave a variety of cellular substrates after aspartic acid residues - a characteristic that is central to their role in mammalian apoptosis. Caspases are synthesized in the cytosol of mammalian cells as inactive zymogens, which become active through intracellular caspase cascades. The TribioTM Caspase-3 fluorometric Assay...

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Caspase-1 Colorimetric Assay kit

Apoptosis plays a fundamental role in many normal biological processes as well as in several disease states. Apoptosis can be induced by various stimuli that all produce the same end result: systematic and orderly cell death. The inflammasome is a large multiprotein complex whose assembly leads to the activation of caspase-1, which promotes the maturation of proinflammatory cytokines interleukin-1β (IL-1β) and IL-18. The TriboTM Caspase-1 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that...

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Boron Colorimetric Assay

Boron is an essential micronutrient in plants and is involved in maintaining robust cell walls, cell membranes, and reproductive tissues. Although boron is common in the soil in its natural state as a borate mineral, the amount of boron available to plants is quite small. As a result, boron deficiency is the second most common micronutrient deficiency among crop plants. In order to keep plant boron levels in a healthy range, supplementation to the soil via fertilizers and additives is...

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0.5M TCEP-HCl Solution (pH 6.0, 7.0 and 8.0)

Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) Solution is an extraordinarily powerful, odorless, thiol-free reducing agent with broad application to protein and nucleic acid involving reduction of disulfide bonds. The pH value of the solution is designed at 6.0, 7.0, and 8.0 for multiple choices. This product is an effective and convenient replacement for βmercaptoethanol (β-ME) or DTT in protein analysis. 0.5M TCEP-HCl Solution (pH 6.0, 7.0 and 8.0) (TBS5082) Related Products: Urea Solution (8M) (TBS5037)DTT Solution (1M) (TBS5039)MOPS Buffer (1M) (TBS5041)Sodium Citrate Solution (1M) (TBS5043)Ammonium...

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40% Deionized Glyoxal Solution

40% Deionized Glyoxal Solution(6M) is designed for RNA denaturation compatible with all buffered agarose gels. Following electrophoresis, RNA can be visualized with Nucleic Acid Stain. Glyoxal denaturation makes the band sharper than formaldehyde in Northern Blot. 40% Deionized Glyoxal Solution (TBS6023) Related Products: 10x DNA Loading Buffer (TBS5018)DEPC-Treated H2O (TBS5036)MOPS Buffer (1M) (TBS5041)Cell RNA Isolation Kit (TBS6001)Saline-Sodium Citrate (SSC-20x pH 7.0) (TBS5033)One-Step TaqProbe qRT-PCR Kit (TBS4008)

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Resazurin Cell Viability Kit

Resazurin Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. It uses the indicator dye resazurin to measure the metabolic capacity of cells an indicator of cell viability. Viable cells retain the ability to reduce resazurin into resorufin, which is highly fluorescent. Nonviable cells rapidly lose metabolic capacity, do not reduce the indicator dye, and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in a...

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Total Aflatoxin Fast ELISA

Aflatoxins are a class of structurally similar mycotoxins produced by Aspergillus species. Currently, 17 different types of aflatoxins have been discovered, including aflatoxins B1 and B2 (produced by A. flavus and A. parasiticus), aflatoxins G1 and G2 (produced by A. parasiticus), and aflatoxins M1 and M2, metabolites of B1 and B2 respectively. Total Aflatoxin, the most common form of aflatoxin originated in naturally contaminated food, has acute toxicity to animals, and has been classified as a Group 1 carcinogen by...

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