Author - Tribioscience
21Jul

Resazurin Cell Viability Kit

Resazurin Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. It uses the indicator dye resazurin to measure the metabolic capacity of cells an indicator of cell viability. Viable cells retain the ability to reduce resazurin into resorufin, which is highly fluorescent. Nonviable cells rapidly lose metabolic capacity, do not reduce the indicator dye, and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in a...

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16Jul

Total Aflatoxin Fast ELISA

Aflatoxins are a class of structurally similar mycotoxins produced by Aspergillus species. Currently, 17 different types of aflatoxins have been discovered, including aflatoxins B1 and B2 (produced by A. flavus and A. parasiticus), aflatoxins G1 and G2 (produced by A. parasiticus), and aflatoxins M1 and M2, metabolites of B1 and B2 respectively. Total Aflatoxin, the most common form of aflatoxin originated in naturally contaminated food, has acute toxicity to animals, and has been classified as a Group 1 carcinogen by...

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12Jul

Fast qPCR Master Mix

TriboTM 2x Fast Sybr Green qPCR Master Mix with different ROX levels and package sizes provide a convenient choice suitable for different qPCR instruments. It is a 2x concentrated premix containing Taq DNA polymerase, dNTPs, Mg2+, SYBR Green I dye (detection), ROX reference dye, and PCR buffer components. The proprietary chemical modifications of the enzyme make PCR hot start activation much faster (less than 1 minute) than the normal 10-minute activation in standard real-time qPCR amplifications. This confers a significant...

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23Jun

Tribo™ Human IFN γ ELISA Kit

Interferon-gamma (IFN-γ, also known as type II interferon) is an important immunoregulatory cytokine through its antiviral activity. IFN- γ is produced by a number of cell types under inflammatory conditions, including dendritic epidermal cells, T cells, keratinocytes, peripheral blood T cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, NK cells, CD4+T cells, and testicular spermatids. It plays a key role in host defense, and the progression of inflammatory diseases such as autoimmunity and atherosclerosis by exerting anti-viral, anti-proliferative,...

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18Jun

XTT Cell Viability Assay

TribioScience’s XTT Cell Viability Assay Kit is a colorimetric assay to quantify the viable cells with soluble formazan dye. The formation of orange formazan is based on the reduction of yellow tetrazolium salt (XTT) under the facilitation of electron acceptors or electron coupling reagents with mitochondrial oxidoreductases that only existed in the living cells. The orange formazan dye is soluble in the liquid solution, can be directly quantified by measuring the absorbance at 450 nm wavelength. The assay can be...

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18Jun

MTT Cell Viability Assay

MTT assay is one of the most versatile and popular assays. It is based on the cleavage of the yellow tetrazolium salt MTT to purple formazan crystal by metabolic active cells. The formazan is then solubilized, and the concentration determined by optical density at 570 nm. The formazan color intensity is directly proportional to the number of living cells in the culture. The MTT Cell Viability Assay Kit provides a convenient, sensitive, quantitative, and reliable assay method for the determination of...

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16Jun

LDH Cytotoxicity Assay

Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme which is present in most cells. It is released upon damage of the cytoplasmic membrane. The measurement of LDH release is a well-accepted assay to estimate cell membrane integrity and quantify cell cytotoxicity. The Tribo™ LDH Cytotoxicity Assay Kit uses resazurin as a fluorogenic indicator for measuring the activity of LDH released from damaged cells (Scheme 1). The fluorescent signal is proportional to the number of dead cells. The kit provides an easy...

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15Jun

WST-8 or Cell Count Kit-8 (CCK-8)

TribioScience’s Cell Counting Kit-8 (CCK-8) is a colorimetric assay to quantify the viable cells, consisting of the tetrazolium salt and the electron mediator 1-mthoxyPMS. The working principle is based on dehydrogenase activity detection. The generated NADH from the reaction of dehydrogenase enzymes and the substrates oxidizes the 1-methoxyPM. The reduced 1- methoxyPMS further oxidizes the tetrazolium salt (WST-8) in the CCK-8 assay kit, forming the yellow formazan dye. The dye color can then be quantified by measuring the absorbance at...

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27May

β-Hexosaminidase Activity Assay

β-Hexosaminidase (β-N-Acetylglucosaminidase, NAGase, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidneys, liver, lungs, and mast cells. Elevated enzyme levels are associated with many disorders, such as TaySachs and Sandhoff disease, inflammation, abnormal immune responses. Related Products: Tribo™ Human IFN γ ELISA Kit (TBS3230)Tribo™ Tryptase Activity Assay (TBS2101)Cytochrome C Oxidase Activity Assay (TBS2115)

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