Fast Prolactin/PRL ELISA, 1step and 1hr (TBS3285)

$560.00

Cell culture Supernate, Plasma/Serum PRL

SKU: TBS3285 Category:
SKUStock SIZE PriceQuantity
TBS3285 Yes 1 x 96-well plate $560.00
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Description

The Fast Human PRL ELISA is a solid phase ELISA designed to measure human PRL levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, with no need for 4-5 hours (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of human PRL samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human PRL protein.

 

Typical Data

This standard curve (R2=0.9925) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human PRL is typically 3.0 pg/ml.

The Intra-assay CV is 4.9% the Inter-assay CV is 8.1%.

Specificity

This assay recognizes natural and recombinant human PRL.

 

Simple Procedures

TBS3235-0

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Human PRL Microplate TBS3285A 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human PRL. The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human PRL Standard TBS3285B 30 µl of Recombinant human PRL protein (50ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3285C 2.1 ml of human PRL antibody. May be stored for up to 3 months at 2-8 °C.
Detection B TBS3285D 12 ml of Streptavidin-HRP
Assay Diluent TBS3285E 12 ml of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

Additional information

SIZE

1 x 96-well plate

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