Tribo™ Reverse Transcription Reaction Kit (TBS4006)

Description

Tribo™ Reverse Transcription Kit is optimized to synthesize first-strand cDNA for PCR or qPCR. The optimal first-strand cDNA synthesis temperature is 50°C. The reverse transcription reaction at higher temperatures improves cDNA yields for difficult RNA transcription. This pre-mixed 5X concentrated solution includes all necessary components for first-strand synthesis. With the mixture of 5X RT buffer, dNTPs, MgCl₂, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, and Reverse Transcriptase, the first-strand cDNA synthesis process is greatly simplified.

Applications:

This kit can be used for first-strand cDNA synthesis.

Key Features:

• Easy-to-use pre-mixed package (All in One)
• High fidelity, yield, and sensitivity
• Broader linear range
• Complete cDNA synthesis guaranteed
• Robust and high throughput
• Work with difficult sequences

Kit Contents:

5x RT reagent: 0.2mL
The kit contains RT buffer, dNTPs, DTT, RNase inhibitor, Primer mixer, and Reverse Transcriptase. The reagent is enough for 50 RT reactions in 20μL.

Storage Conditions:

The kit is shipped on ice. All components should be stored at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receiving.

Research use only.

Additional information

Datasheet:

Reverse Transcription Reaction Kit

MSDS:

TBS4006_Reverse_Transcription_Reaction_Kit_MSDS-2024

Research use only.

1. Ramos-León, J., Valencia, C., Gutiérrez-Mariscal, M., Rivera-Miranda, D.-D.-A., García-Meléndrez, C., & Covarrubias, L. (2023, March 09). The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism. bioRxiv. https://www.biorxiv.org/content/10.1101/2023.03.09.531979v1

2. Ramos-León, J., Valencia, C., Gutiérrez-Mariscal, M., Rivera-Miranda, D. A., García-Meléndrez, C., & Covarrubias, L. (2024). The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism. Experimental cell research, 437(1), 113965. https://doi.org/10.1016/j.yexcr.2024.113965

3. Arjavalingam, A. (2022). Modeling Neuronal Circadian Rhythms in Major Depressive Disorder using Human Induced Pluripotent Stem Cells. UC San Diego.  https://escholarship.org/uc/item/9kf1j00p

Research use only.