Human CD30/TNFRSF18 ELISA (TBS32109)
$560.00
SKU | Stock | SIZE | Price | Quantity | ||
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TBS32109 | Yes | 1 x 96-well plate | $560.00 |
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Description
Tribioscience’s Fast Human CD30 ELISA is designed to quantitatively detect human CD30 levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 7 to 2000 pg/mL. The levels of human CD30 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human CD30 protein.
Typical Data
This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human CD30 is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human CD30. No cross-reactivity to others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part | Part# | Description | Storage of Opened/Reconstituted |
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Human CD30 Microplate | TBS32109A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for Human CD30. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
Human CD30 Standard | TBS32109B | 20 µL of Recombinant Human CD30 protein (100 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS32109C | 2.2 mL of Biotin-Human CD30 antibody. | May be stored for up to 3 months at 2-8 °C. |
Detection B | TBS32109D | 200 µL of Streptavidin-HRP. | |
Assay Diluent | TBS32109E | 25 mL of a buffered protein base with preservatives. | |
Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Additional information
SIZE | 1 x 96-well plate |
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