Water-soluble XTT
Water-soluble XTT
Water-soluble XTT offers a significant advantage in cell viability assays by eliminating the need for additional solubilization steps, streamlining the workflow for researchers. TribioScience’s XTT Cell Viability Assay Kit utilizes a specially formulated XTT reagent that dissolves readily in aqueous solutions, forming a clear working solution when mixed with an activator at a simple 1:50 ratio. Upon contact with metabolically active cells, the yellow XTT salt is reduced to a bright orange formazan dye through mitochondrial oxidoreductase activity, a process exclusive to viable cells. Since this formazan is water-soluble, absorbance can be measured directly in the assay plate at 450 nm, simplifying analysis and minimizing handling errors.
This water-soluble XTT formulation enhances experimental accuracy and throughput by removing traditionally required wash or cell transfer steps. The entire assay can be conducted in a single 96-well plate, where researchers add the XTT working solution directly to cultured cells and incubate for just 2–4 hours. Because the dye remains in solution, there’s no need for organic solvents or detergents to solubilize the formazan, reducing the risk of variability and contamination. As a result, this assay is ideal for high-throughput screening of cytotoxic agents, anticancer drugs, and other pharmaceutical compounds, delivering reliable and reproducible results with minimal user intervention.
Moreover, the XTT Cell Viability Assay is designed for versatility across a broad range of biological applications, including monitoring cell proliferation, testing drug reagents, evaluating growth factor responses, and assessing apoptosis. Its non-radioactive and straightforward procedure ensures both user safety and convenience, making it suitable for routine use in research and drug development settings. The assay kit, stable for 12 months when stored at -20°C, empowers scientists to efficiently quantify living cells and make critical decisions based on accurate cell viability measurements — all facilitated by its water-soluble XTT chemistry.
