Insulin HEPES buffer

Insulin HEPES buffer

Formulated in 25 mM HEPES buffer, Tribioscience’s bovine insulin solution is designed to deliver consistent pH stability, a critical factor for maintaining sensitive cell culture environments. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a widely used biological buffer that effectively stabilizes pH without interfering with biological reactions. By combining this buffering capability with high-quality bovine insulin, the solution ensures optimal conditions for cell viability, differentiation, and growth in a wide range of in vitro applications.

Bovine insulin closely mirrors human insulin, differing by only three amino acid residues, and retains its biological activity in human-related systems. This high degree of similarity makes bovine insulin a reliable and cost-effective substitute in diabetes research, islet transplantation studies, and routine cell culture use. When prepared in a 25 mM HEPES buffer, the insulin remains stable and bioactive, supporting its role as a potent growth supplement in cell culture media at concentrations ranging from 1 to 10 µg/mL.

Our insulin HEPES buffer formulation is available in convenient pack sizes of 1 mL, 2 mL, and 5 mL, with each bottle containing 10 mg/mL of bovine insulin. This cell culture-grade product is ready to use and compatible with a variety of cell types, making it an ideal choice for researchers seeking consistency and performance. The precise formulation helps improve experimental reproducibility and cellular outcomes.