Ready-to-Use Hybridoma Medium

Ready-to-Use Hybridoma Medium

The Ready-to-Use Hybridoma Medium with HT offers a highly convenient and reliable solution for researchers engaged in hybridoma cell culture and monoclonal antibody production. As a pre-prepared, chemically defined liquid medium, it eliminates the need for time-consuming media preparation and minimizes the risk of human error. By delivering batch-to-batch consistency, this medium ensures reproducible results that are critical for downstream applications such as antibody screening and selection.

Formulated specifically to support hybridoma expansion following HAT selection, this medium contains hypoxanthine and thymidine (HT), essential for weaning hybridomas off aminopterin. Its animal component–free formulation makes it suitable for sensitive research applications, including those requiring stringent control over input variables. The inclusion of L-glutamine, gentamicin, and phenol red supports robust cell growth, pH stability, and protection against bacterial contamination. Verified for use in both mouse and rat hybridoma systems, the medium is optimized for high cell yield and consistent monoclonal antibody production.

For added convenience, the medium comes in a 500 mL unit size and is easy to store and use. It can be thawed at room temperature or overnight at refrigerated conditions and stored at 2–8°C for up to two weeks once opened, or kept frozen at -20°C for long-term use. These user-friendly handling and storage conditions, combined with the medium’s consistent performance, make it an essential component for any lab engaged in hybridoma development or antibody generation.