Hybridoma Media

Hybridoma Media

Hybridoma Media is specifically formulated to support the unique needs of hybridoma cells during and after the critical post-HAT (hypoxanthine-aminopterin-thymidine) selection phase. This tailored growth medium delivers the essential nutrients required for sustained hybridoma cell viability, proliferation, and high monoclonal antibody yield. Its formulation, which includes hypoxanthine and thymidine (HT), ensures a seamless transition for hybridomas as they are weaned off aminopterin, promoting continued expansion without compromising genetic stability or productivity.

The medium is a chemically defined, animal component-free solution, ideal for researchers seeking consistent and reproducible results. Key components such as L-glutamine, gentamicin, and phenol red support optimal cell health and monitoring, while the absence of serum and animal-derived additives minimizes variability and the risk of contamination. Designed for use with both mouse and rat hybridoma cells, this media has been thoroughly validated for robust monoclonal antibody (MAb) production, making it a reliable choice for downstream therapeutic and diagnostic antibody research.

Hybridoma Growth Medium is conveniently supplied in 500 mL bottles and offers flexibility in storage and handling. It can be thawed at room temperature or overnight at 2–8°C, and once thawed, can be stored refrigerated for up to two weeks or aliquoted and refrozen at -20°C until its one-year expiry. This user-friendly medium streamlines laboratory workflow, supports high cell yields, and ensures consistent performance across hybridoma culture systems, from early-stage expansion to large-scale antibody production.