Hybridoma Culture Growth Medium

Hybridoma Culture Growth Medium

Hybridoma Culture Growth Medium is specifically formulated to address the distinct metabolic and proliferation requirements of hybridoma cells following HAT (hypoxanthine, aminopterin, thymidine) selection. This chemically defined liquid medium contains hypoxanthine and thymidine (HT), essential for transitioning hybridoma cells away from the selective pressure of aminopterin, allowing for stable cell growth and viability. The precise composition ensures that hybridomas are supported through the critical weaning phase, promoting a smooth shift into sustained culture conditions conducive to monoclonal antibody (MAb) production.

Engineered to maximize hybridoma expansion and antibody yield, the medium’s formulation is free of animal-derived components, reducing variability and potential contaminants. Key ingredients such as L-glutamine, gentamicin, and phenol red contribute to a balanced nutritional environment. These features make it an ideal choice for researchers and biomanufacturers seeking reproducible results and robust hybridoma cultures, particularly in mouse and rat monoclonal antibody development workflows.

To preserve the integrity and performance of the medium, it is supplied in a 500 mL unit and designed for long-term storage at -20°C for up to one year. Upon thawing, it remains stable for short-term use at 2–8°C for up to two weeks, ensuring flexibility in experimental planning. Whether used immediately after HAT selection or in scaled-up antibody production, Hybridoma Culture Growth Medium delivers optimal support for cell viability, consistent antibody expression, and high-yield performance in chemically defined, animal-free conditions.