HT-Supplemented Medium

HT-Supplemented Medium

HT-Supplemented Medium is specially formulated for optimal use in mouse and rat hybridoma development, particularly following HAT (hypoxanthine-aminopterin-thymidine) selection. This medium supports the crucial transition phase in hybridoma culture, enabling effective screening and stable expansion. By eliminating aminopterin while maintaining hypoxanthine and thymidine, the medium facilitates the selective survival and growth of hybridoma cells, making it ideal for monoclonal antibody production workflows.

The formulation is chemically defined and free from animal-derived components, providing consistent performance and reduced variability. Its key components—hypoxanthine, thymidine, L-glutamine, phenol red, and gentamicin—create an environment optimized for high hybridoma viability and productivity. This medium is validated for both mouse and rat hybridomas, making it a reliable choice for researchers engaged in generating high-yield monoclonal antibodies across multiple model systems.

With its proven support for hybridoma expansion after HAT selection, this HT-supplemented medium is an essential tool for immunology and biotechnology labs. Available in a convenient 500 mL format and stored at -20°C with a shelf life of one year, it offers both reliability and ease of use in hybridoma culture applications. Whether you’re scaling up antibody production or stabilizing new clones, this medium ensures efficient growth and consistent results.