HT Hybridoma Media for Cloning

HT Hybridoma Media for Cloning

HT Hybridoma Media for Cloning is specifically optimized to support the selective growth and enhanced stability of hybridoma clones of monoclonal antibody (mAb) development. This chemically defined, animal component-free medium is formulated to facilitate the expansion of hybridoma cells after hypoxanthine-aminopterin-thymidine (HAT) selection. By including hypoxanthine and thymidine (HT), the medium gently weans cells off aminopterin, ensuring a smoother transition while maintaining selective pressure, ultimately supporting the viability and genetic stability of emerging clones.

The medium is verified for use in both mouse and rat hybridoma systems, ensuring broad compatibility for mAb production workflows. Its composition includes essential ingredients such as hypoxanthine, thymidine, gentamicin, phenol red, and L-glutamine, along with other optimized nutrients that promote high cell yield and antibody expression. Designed for consistency and reproducibility, this formulation eliminates variability associated with serum or animal-derived components, which is critical in regulated and scalable bioproduction environments.

HT Hybridoma Media for Cloning comes in a 500 mL ready-to-use format and is stored at -20°C for long-term stability. Upon thawing at room temperature or overnight refrigeration, the medium supports up to two weeks of storage at 2–8°C, making it highly convenient for laboratory workflows. Whether used immediately after HAT selection or for downstream clone stabilization, this medium ensures a robust platform for generating high-quality monoclonal antibodies with consistent performance across development stages.