Cell Media with HT Supplement

Cell Media with HT Supplement

Cell Media with HT Supplement is a specialized, ready-to-use culture medium designed to support selective cell culture applications, particularly the growth and expansion of hybridomas and other HT-dependent cell lines. This chemically defined formulation includes essential nutrients—hypoxanthine and thymidine (HT)—which are crucial for maintaining the viability and productivity of cells following HAT (hypoxanthine-aminopterin-thymidine) selection. By omitting aminopterin and including only HT, the media provides a transition platform that facilitates robust hybridoma survival and proliferation after the selection phase.

This medium is free of animal-derived components and is engineered for high cell yield and monoclonal antibody (MAb) production, making it ideal for bioproduction environments. It has been validated for use with both mouse and rat hybridoma lines, ensuring broad compatibility in immunology and biotechnology applications. In addition to HT, the formulation includes gentamicin to prevent bacterial contamination, L-glutamine for optimal cell metabolism, and phenol red as a pH indicator, providing a stable and controlled environment conducive to consistent cell growth.

Available in a 500 mL unit, the Hybridoma Growth Medium with HT offers convenient storage and usage options. It should be stored at -20°C for up to one year, and once thawed, can be kept at 2–8°C for up to two weeks. Easy-to-follow preparation guidelines ensure that labs can rapidly integrate the medium into existing workflows without compromising performance. Overall, Cell Media with HT Supplement offers a reliable, defined, and high-performance solution for researchers focused on hybridoma development and antibody production.