Cell Growth Medium for Hybridoma

Cell Growth Medium for Hybridoma

The Cell Growth Medium for Hybridoma is nutritionally optimized to meet the unique metabolic needs of hybridoma cell lines, particularly during the critical post-HAT selection stage. This specialized medium supports rapid cell division and robust cell expansion, providing a chemically defined formulation that eliminates variability and ensures consistency. Enriched with hypoxanthine and thymidine (HT), the medium effectively facilitates the weaning process from aminopterin, enabling the smooth transition of hybridoma cells while preserving their viability and productivity.

Engineered for high-performance monoclonal antibody (MAb) production, the medium is free from animal-derived components, reducing the risk of contamination and making it suitable for regulated and sensitive workflows. Its formulation includes essential nutrients such as L-glutamine for energy metabolism, as well as gentamicin for microbial protection and phenol red for pH monitoring. These components collectively foster a stable environment that encourages sustained cell health and high-yield antibody expression, which is crucial for scalable production in both research and industrial applications.

Validated for use with both mouse and rat hybridoma cell lines, the Hybridoma Growth Medium with HT has proven effective in supporting long-term culture viability and productivity. Supplied in a 500 mL liquid format and stable for up to one year when stored at -20°C, it is an ideal solution for labs focused on generating consistent, high-quality monoclonal antibodies. This medium is particularly recommended during the hybridoma expansion phase following HAT selection, ensuring smooth cellular adaptation and peak performance throughout the production cycle.