Cell Culture Medium HT

Cell Culture Medium HT

Cell Culture Medium HT is a chemically defined, nutrient-rich formulation specifically designed to support the growth and productivity of cell lines that rely on the salvage pathway for nucleotide synthesis. It contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin following the selection process. This medium is particularly effective during the post-selection phase after hypoxanthine-aminopterin-thymidine (HAT) selection, where aminopterin inhibits the de novo pathway, making the salvage pathway essential for cell survival and proliferation.

The inclusion of HT in the culture medium not only supports cell viability but also enhances monoclonal antibody production. Studies have demonstrated that supplementing cultures with hypoxanthine and thymidine can stimulate initial cell growth and increase volumetric antibody production by up to 22%, primarily through elevated integrated viable cell concentration. Furthermore, a biphasic addition strategy—introducing low concentrations of HT during the initial growth phase and higher concentrations during the production phase—has been shown to improve antibody yields significantly.

Cell Culture Medium HT is formulated without animal-derived components, reducing the risk of contaminants and ensuring consistency across batches. This animal origin-free composition simplifies downstream processing and is suitable for various applications, including hybridoma expansion and monoclonal antibody production. The medium is available in a 500 mL liquid format and should be stored at -20°C for up to one year to maintain its efficacy.