DMEM no glucose no glutamine no phenol red

DMEM no glucose no glutamine no phenol red

Dulbecco’s Modified Eagle Medium (DMEM) is a basal cell culture medium designed to support the growth and maintenance of many mammalian cell types. It contains higher levels of amino acids and vitamins than Eagle’s Minimal Essential Medium (EMEM), providing a more nutrient-rich foundation. DMEM is typically supplemented with Fetal Bovine Serum (FBS) or other additives depending on the cell type and experiment.

This formulation is modified without glucose, L-glutamine, and phenol red, allowing precise control over metabolic and experimental conditions. It provides a defined basal medium that can be supplemented according to specific cell culture requirements.

The medium includes sodium bicarbonate to help maintain proper pH in CO₂ incubators. When supplemented appropriately, it can support the culture of various primary cells and cell lines, including fibroblasts, neurons, endothelial cells, smooth muscle cells, and commonly used lines such as HeLa and 293.