Neutral Protease, Grade II
Neutral Protease, Grade II
Dispase II (Neutral Protease, Grade II) is a high-quality neutral protease renowned for its gentle yet effective cell dissociation properties. It hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues, enabling the separation of cells and tissues without compromising cell membrane integrity. This mild enzymatic action makes Dispase II ideal for applications requiring viable, intact cells, such as primary cell isolation and tissue engineering.
When used in vitro, Dispase II (Neutral Protease, Grade II) efficiently detaches adherent cells by selectively cleaving extracellular matrix proteins such as fibronectin and type IV collagen, while leaving laminin, type V collagen, serum albumin, and transferrin unaffected. This specificity preserves the extracellular environment and supports high yields of healthy, functional cells.
In suspension cultures, Dispase II (Neutral Protease, Grade II) also minimizes cell clumping, maintaining uniform cell suspensions and optimal growth conditions. Its gentle dissociation properties and high purity make it a preferred reagent for researchers who require reliable performance with minimal impact on cell surface proteins and overall cell physiology.
