Enzyme for Separating Tissues and Cells
Enzyme for Separating Tissues and Cells
Dispase II is a gentle, neutral protease widely used for the efficient separation of tissues and cells in vitro. It functions by hydrolyzing the N-terminal peptide bonds of non-polar amino acid residues, effectively loosening the extracellular matrix without damaging cell membranes. This mild enzymatic action allows for the release of intact, viable cells suitable for further culture or experimental applications.
One of the key advantages of Dispase II is its ability to maintain cell membrane integrity during dissociation. Unlike harsher enzymes, it provides a rapid yet gentle separation process, minimizing cellular stress and preserving essential surface proteins. Derived from a non-mammalian source, it is free of mycoplasma and animal virus contamination, ensuring a high degree of purity and biosafety in cell culture workflows.
Additionally, Dispase II offers excellent stability across a wide range of temperature and pH conditions, and it remains active even in the presence of serum components. The enzyme can be easily inactivated by chelating agents or simple dilution, allowing precise control over the dissociation process. Its combination of high activity, stability, and convenience makes Dispase II an ideal choice for applications requiring gentle, efficient cell isolation.
