Dispase II (TBS2117-01)

Dispase II (TBS2117-01)

Dispase II (TBS2117-01) from Tribioscience is a high-quality neutral protease with an activity of 1.0 U/mg, designed to provide gentle and effective tissue dissociation. This enzyme specifically hydrolyzes N-terminal peptide bonds of non-polar amino acid residues, making it ideal for separating a wide range of tissues and cells grown in vitro without compromising cell viability. Its mild action preserves cell membrane integrity, enabling researchers to obtain high yields of healthy, functional cells for culture and analysis.

With its gentle enzymatic activity, Dispase II (TBS2117-01) is particularly suited for applications that require precise control during tissue dissociation, such as isolating epithelial cells, embryonic cells, or delicate primary cultures. It also helps prevent cell clumping in suspension cultures, improving the consistency and reliability of experimental results. Tribioscience’s optimized formulation ensures consistent performance and reproducible outcomes across various tissue types and culture conditions.

For best results, tissue samples are minced, washed, and incubated in a Dispase solution (0.6–2.4 U/mL) at 37°C, allowing for gradual and complete dissociation without harming the cells. The process can be enhanced by combining Dispase II with collagenase for more efficient breakdown of compact tissues. With its high purity, gentle action, and proven reliability, Tribioscience’s Dispase II (TBS2117-01) is an essential reagent for researchers seeking effective tissue dissociation.