Beta-NAG High-throughput screening

Beta-NAG High-throughput screening

The β-N-Acetylglucosaminidase (β-NAG) Activity Fluorometric Assay is specifically designed for high-throughput screening (HTS), enabling rapid and reliable quantification of β-NAG activity across thousands of biological samples daily. This lysosomal enzyme, expressed in organs such as the kidneys, liver, lungs, and mast cells, plays a key role in several physiological and pathological conditions, including Tay-Sachs and Sandhoff diseases, as well as immune and inflammatory disorders. The assay leverages a sensitive fluorescence-based detection method using the non-fluorescent substrate 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUAG), which, upon enzymatic cleavage, releases the highly fluorescent 4-Methylumbelliferone (4-MU). Fluorescence is then quantified at an excitation of 365 nm and an emission of 445 nm.

Tailored for high-throughput workflows, this assay supports seamless integration with automated liquid handling systems. It is optimized for speed and sensitivity, delivering a linear detection range within a 30-minute reaction at 37°C using just 20 µL of sample. This efficiency allows researchers to rapidly process and analyze large sample sets from tissues, cells, serum, or urine with consistent and reproducible results. The assay’s design supports streamlined execution through multi-channel pipettors, facilitating uniform substrate addition and reaction termination, which are critical for kinetic-based fluorescence assays.

The β-NAG assay kit includes all essential reagents—substrate, standard, positive control, stop reagent, and buffer—conveniently packaged for 100x or 200x reaction formats. Its long shelf life (12 months when stored at -20°C) and compatibility with HTS platforms make it an ideal solution for laboratories engaged in large-scale screening efforts, including drug discovery, disease biomarker validation, and mechanistic enzymology studies. By combining precision, scalability, and ease of use, the β-NAG High-throughput Screening Assay empowers researchers to accelerate their investigations into lysosomal function and related pathologies.