Rapid ADP measurement

Rapid ADP measurement

Achieving rapid ADP measurement in minutes is critical for high-throughput screening (HTS) applications, and the ADP Colorimetric/Fluorometric Assay Kit (TBS2020) from Tribioscience is designed to meet this need. This assay enables detection of ADP concentrations as low as 1 µM in various biological samples, utilizing a straightforward “load and incubate” procedure that takes less than 60 minutes. By converting ADP to ATP and pyruvate, the assay allows for quantification through either colorimetric (absorbance at 570 nm) or fluorometric (excitation/emission at 530/590 nm) methods, providing flexibility and sensitivity for different experimental setups.

The TBS2020 kit is particularly well-suited for HTS due to its compatibility with 96-well plate formats and its minimal sample requirement of just 10 µL. The assay’s simplicity and adaptability make it ideal for processing large numbers of samples efficiently, which is essential in drug discovery and enzyme activity studies. Furthermore, the kit’s components are stable when stored at -20°C, ensuring consistent performance across multiple assays.

Incorporating the TBS2020 assay into your workflow can streamline the process of ADP measurement, providing reliable and rapid results that are crucial for HTS applications. Its ease of use, sensitivity, and compatibility with standard laboratory equipment make it a valuable tool for researchers aiming to accelerate their screening processes and obtain accurate data in a time-efficient manner.