Easy-to-use ADP assay
Easy-to-use ADP assay
The Tribioscience ADP Colorimetric/Fluorometric Assay Kit (TBS2020) is designed to be user-friendly, making it ideal for both novice users and experienced laboratory professionals. Its straightforward “load and incubate” protocol requires minimal hands-on time and can be completed in under 60 minutes. The assay involves converting ADP to ATP and pyruvate, with the generated pyruvate quantified either colorimetrically at 570 nm or fluorometrically at Ex/Em 530/590 nm. This flexibility allows users to choose the detection method that best suits their available equipment and sensitivity requirements. The kit’s simplicity ensures that even those new to biochemical assays can achieve reliable results without extensive training.
For seasoned researchers, the TBS2020 kit offers high sensitivity and adaptability for various experimental needs. It can detect ADP concentrations as low as 1 µM using just 10 µL of sample, making it suitable for analyzing limited or precious biological specimens. The assay is compatible with a wide range of sample types, including cell and tissue lysates, culture media, urine, plasma, and serum. Its design supports high-throughput applications, allowing for efficient processing of multiple samples in a 96-well plate format. This adaptability makes it a valuable tool for diverse research areas, from metabolic studies to drug screening.
The kit’s stability and storage conditions further enhance its practicality in the laboratory setting. All components are stored at -20°C, with a shelf life of three months, ensuring readiness for use over extended periods. Before use, components (except enzymes) should be warmed to room temperature, and small vials briefly centrifuged to ensure proper mixing. These straightforward preparation steps contribute to the assay’s ease of use and reliability. Overall, the Tribioscience ADP Colorimetric/Fluorometric Assay Kit combines simplicity, sensitivity, and versatility, making it a dependable choice for researchers at all experience levels.
