ADP detection kit

ADP detection kit

The Tribioscience ADP Colorimetric/Fluorometric Assay Kit (TBS2020) offers a sensitive and convenient solution for quantifying adenosine diphosphate (ADP) in various biological samples. By converting ADP to ATP and pyruvate, the kit enables detection through either colorimetric (absorbance at 570 nm) or fluorometric (excitation/emission at 530/590 nm) methods. This dual-detection capability enhances flexibility in experimental design, catering to diverse laboratory workflows. The assay is capable of detecting ADP concentrations as low as 1 µM, making it suitable for applications requiring high sensitivity.

Designed for streamlined laboratory procedures, the TBS2020 kit simplifies the ADP quantification process. The protocol involves minimal steps: sample preparation, addition of the reaction mix, incubation, and measurement—all achievable in under 60 minutes. With a detection range of 1–1000 µM and requiring only 10 µL of sample per well in a 96-well plate format, the kit is well-suited for high-throughput analyses. This efficiency reduces hands-on time and potential for error, facilitating consistent and reproducible results across multiple assays.

The kit includes all necessary reagents: ADP assay buffer, probe, substrate, enzyme mix, and a 50 mM ADP standard, sufficient for 100 tests. Components are stable when stored at -20°C, with a shelf life of three months, ensuring readiness for use over extended periods. The assay’s adaptability to both colorimetric and fluorometric detection methods allows integration into various analytical platforms, enhancing its utility in research focused on cellular metabolism, enzyme activity, and energy transfer processes.