HEPES buffered saline 2x
HEPES buffered saline 2x
HEPES buffered saline 2x (2x HBS) is a ready-to-use solution specifically designed to provide an optimal environment for calcium phosphate transfection methods. Its precise composition of 280 mM NaCl, 50 mM HEPES buffer, and 1.42 mM Na₂HPO₄·7H₂O, adjusted to a stable pH of 7.05, ensures consistent and reproducible conditions across experiments. By maintaining a balanced physiological environment, 2x HBS enhances the efficiency of DNA uptake in mammalian cells, making it a valuable reagent in both research and production settings.
One of the key advantages of using HEPES buffered saline 2x lies in its ability to support reproducible results in mammalian cell and virus production workflows. The solution is sterile filtered through a 0.2 μm filter, reducing the risk of contamination and ensuring reliability in sensitive transfection protocols. When paired with calcium chloride solutions (1.25 M or 2.5 M), 2x HBS facilitates the formation of calcium phosphate-DNA complexes that efficiently enter cells, a critical step in applications such as lentivirus, retrovirus, and AAV transfection.
In addition to its performance benefits, 2x HBS offers convenience and stability. Supplied in a 500 mL unit size, the solution is easy to integrate into laboratory workflows and can be stored at room temperature (18–25°C) for up to 12 months after receipt without compromising quality. Its versatility makes it suitable for a wide range of transfection applications, including chemical and calcium phosphate transfection, mammalian cell transfection, and viral vector production. With its carefully optimized formulation, HEPES buffered saline 2x provides a dependable foundation for high-efficiency and reproducible transfection experiments.
