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Tribo™ Human IFN γ ELISA Kit

$390.00

The Tribo™ Fast Human IFN-γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma.

SKU: TBS3230 Category: Tag:
SKUStock SIZE PriceQuantity
TBS3230 Yes 1 x 96-well plate $390.00
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Description

The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The measurement can be finished within 1.0 hour, with no need for 4-5 hours. The detection range is from 20 to 15000 pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.

Sensitivity

The minimum detectable dose (MOD) of human IFN-γ is typically 8 pg/ml.

Specificity

This assay recognizes natural and recombinant human IFN- γ.

 

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Kit Contents & Storage Conditions

Part Part # Description Storage of Opened/Reconstituted
Human IFN-γ Microplate TBS3230A 96-well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN- γ. Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human IFN-γ Standard TBS3230B 100 µL of Recombinant human IFN- γ protein (145.8ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3230C 2.2 ml of HRP-Human IFN- γ antibody. May be stored for up to 3 months at 2-8℃
Assay Diluent TBS3230D 12 ml of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 ml of concentrated solution (10x).
TMB Substrate TBS3000T 12 ml of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 ml of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Product

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  2. Human IL-2 ELISA (TBS3220)
  3. Human IL-4 ELISA (TBS3221)
  4. Human IL-6 ELISA (TBS3223)
  5. Human IL-7 ELISA (TBS3224)

Additional information

SIZE

1 x 96-well plate

Ao, Z., Wu, Z., Cai, H., Hu, L., Li, X., Kaurich, C., Chang, J., Gu, M., Cheng, L., Lu, X., & Guo, F. (2022). Rapid Profiling of Tumor-Immune Interaction Using Acoustically Assembled Patient-Derived Cell Clusters. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(22), e2201478. https://doi.org/10.1002/advs.202201478

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