DH5α Competent Cell (10×50 uL), >10^9 (TBS8041)

$89.00

SKU: TBS8041-10 Category:
SKUStock SIZE (ul) PriceQuantity
TBS8041-10 Yes 10 x 50 uL $89.00
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Description

Tribo™ DH5α Competent Cells are an E. coli strain DH5α that provides high transformation efficiencies. Cells can be used in a wide variety of applications for cloning and subcloning. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening of colonies on plates containing X-gal or Bluo-gal. DH5α competent cells support the replication of M13mp vectors but do not support plaque formation.

 

Applications

  • General cloning and subcloning.
  • Blue-white selection.
  • Plasmid DNA amplification.
  • Plasmid isolation.

 

Transformation Efficiency (cfu/Ug)
>1×10^9

Blue-White Screening
Yes

Reduces Recombination
Yes

Cloning Methylated DNA
No

Improves Plasmid Quality
Yes

recA Deficient:
Yes

Strain
K12

Genotype

F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk-, mk+) phoAsupE44 thi-1 gyrA96 relA1 λ-

 

Storage Conditions

Store the competent cells at -80°C. Shelf life: 6 months.

Additional information

SIZE (ul)

10 x 50 uL

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