DH5α Competent Cell (10×50 uL), >10^9 (TBS8041)
$89.00
SKU | Stock | SIZE (ul) | Price | Quantity | ||
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TBS8041-10 | Yes | 10 x 50 uL | $89.00 |
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Description
Tribo™ DH5α Competent Cells are an E. coli strain DH5α that provides high transformation efficiencies. Cells can be used in a wide variety of applications for cloning and subcloning. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening of colonies on plates containing X-gal or Bluo-gal. DH5α competent cells support the replication of M13mp vectors but do not support plaque formation.
Applications
- General cloning and subcloning.
- Blue-white selection.
- Plasmid DNA amplification.
- Plasmid isolation.
Transformation Efficiency (cfu/Ug)
>1×10^9
Blue-White Screening
Yes
Reduces Recombination
Yes
Cloning Methylated DNA
No
Improves Plasmid Quality
Yes
recA Deficient:
Yes
Strain
K12
Genotype
F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk-, mk+) phoAsupE44 thi-1 gyrA96 relA1 λ-
Storage Conditions
Store the competent cells at -80°C. Shelf life: 6 months.
Additional information
SIZE (ul) | 10 x 50 uL |
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