NNMT Inhibitor Screening Assay (TBS2097)
$520.00
SKU | Stock | SIZE (test) | Price | Quantity | ||
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TBS2097-100 | Yes | 100 Tests | $520.00 |
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Description
NNMT (Nicotinamide N-Methyltransferase) is an enzyme that catalyzes the methylation of nicotinamide and other pyridines using S-adenosyl-L-methionine (SAM) as the methyl group donor to produce SAH and 1-methylnicotinamide. NNMT plays a significant role in the regulation of metabolic pathways. It is expressed at high levels in several kinds of cancers, neurodegenerative diseases, obesity, and diabetes, indicating a potential target for therapy.
Tribioscience’s NNMT inhibitor screening kit is a fluorometric assay that utilizes SAM and nicotinamide as the substrates to generate SAH which is hydrolyzed to form homocysteine. The free thiol group of the homocysteine can be detected with a thiol probe generating an enhanced fluorescent signal at Ex/Em = 400/465 nm. In the presence of an NNMT inhibitor, the fluorescent signal is reduced in concentration dependent manner. The kit provides the easiest, most accurate, and simplest approach for high-throughput screening of NNMT inhibitors.
Fig. 1: Principle of NNMT Inhibitor Screening Assay
Application
- NNMT inhibitor screening assay
Fig. 2. Inhibition of NNMT activity by MNA
Additional information
SIZE (test) | 100 Tests |
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