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LDH Cytotoxicity Assay

Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme which is present in most cells. It is released upon damage of the cytoplasmic membrane. The measurement of LDH release is a well-accepted assay to estimate cell membrane integrity and quantify cell cytotoxicity. The Tribo™ LDH Cytotoxicity Assay Kit uses resazurin as a fluorogenic indicator for measuring the activity of LDH released from damaged cells (Scheme 1). The fluorescent signal is proportional to the number of dead cells. The kit provides an easy...

16 Jun
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WST-8 or Cell Count Kit-8 (CCK-8)

TribioScience’s Cell Counting Kit-8 (CCK-8) is a colorimetric assay to quantify the viable cells, consisting of the tetrazolium salt and the electron mediator 1-mthoxyPMS. The working principle is based on dehydrogenase activity detection. The generated NADH from the reaction of dehydrogenase enzymes and the substrates oxidizes the 1-methoxyPM. The reduced 1- methoxyPMS further oxidizes the tetrazolium salt (WST-8) in the CCK-8 assay kit, forming the yellow formazan dye. The dye color can then be quantified by measuring the absorbance at...

15 Jun
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β-Hexosaminidase Activity Assay

β-Hexosaminidase (β-N-Acetylglucosaminidase, NAGase, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidneys, liver, lungs, and mast cells. Elevated enzyme levels are associated with many disorders, such as TaySachs and Sandhoff disease, inflammation, abnormal immune responses. Related Products: Tribo™ Human IFN γ ELISA Kit (TBS3230)Tribo™ Tryptase Activity Assay (TBS2101)Cytochrome C Oxidase Activity Assay (TBS2115)

27 May
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Featured Microbial Detection for Food Safety Products

RNA and DNA Purification TBS6009 Aspergillus Genomic DNA ExtractionTBS6025 Microbial DNA Magnetic ExtractionTBS6026 Plant DNA Magnetic Extraction Kit305-101 RNA Purification Kit qRT-PCR Gene Detection TBS4006 Reverse Transcription Reaction Kit (All-in-One)TBS4007R One-step Sybr qRT-PCRTBS4008R One-step Taqmam Probe qRT-PCRTBS42021 Aspergillus Flavus Taqman PCR DetectionTBS42022 Aspergillus Fumigatus Taqman PCR DetectionTBS42023 Aspergillus Niger Taqman PCR DetectionTBS42024 Aspergillus Terreus Taqman PCR DetectionTBS42025 4-in-One Aspergillus qPCR (Flavus, Fumigatus, Niger and Terreus)TBS42026 O157H7 E. Coli qPCR KitTBS42027 STEC qPCR KitTBS42028 Salmonella qPCR KitTBS42029 STEC and Salmonella Multiple qPCR KitTBS42031 Listeria...

21 May
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Northern Blot Service

Northern blot is a molecular biological technique first developed in 1977, known for its wide application on gene expression, disease diagnosis, and viral microRNAs detection, etc. It is a classical methodology to characterize and quantitate specific RNA genes in the sample matrix. The northern analysis first starts from RNA extraction from tissues or cells, which are further separated by denaturing agarose gels. The separated RNAs are transferred to a nylon membrane with capillary forces. RNA on the membrane is then...

13 May
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Tribio™ Tryptase Activity Assay

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein of mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases. Related Products: β-Hexosaminidase Activity Assay (TBS2105)Cytochrome C Oxidase Activity Assay (TBS2115)Fast Glucose Determination Assay (TBS2087)

3 May
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Why Some People Are More Susceptible to SARS-CoV-2?

As COVID19 continues to rage across the US and around the world, scientists and physicians continue to be perplexed by why some younger patients with no underlying conditions succumb to SARS-CoV-2. In addition, these victims are disproportionately male. Now a research article published in Science by Bastard et al. may have provided some clues to the mystery. Bastard’s research shows that about 14% of critically ill COVID19 patients have autoantibodies against Type I interferons, namely IFN α2 and IFN ω. A...

17 Nov
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Monitor Cytokine Storms with Cutting-Edge Technology

COVID19 pandemic and CAR-T cell therapy have put Cytokine Release Syndrome (CRS), commonly known as cytokine storm, in the spotlight. Cytokine storms are caused by inflammation-causing cytokines released by activated T cells.  These cytokines include IFNg, TNFα, IL-1, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-13, IL-17A, IL-22, GM-CSF, Granzyme B, and MIP-1α. Therefore, detections of these cytokines are paramount for monitoring cytokine storms. Traditional ELISA requires lengthy incubation periods at different temperatures and multiple wash steps. It is known to be...

20 Oct
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New Products

SPECIAL PROMOTION: 10% Off  CAR-T Induced Cytokine ELISA Kits Fast ELISA Kits for CAR-T Human Cytokines TBS3219: Human IL-1 ß; ELISA; 1 step and 1hr TBS3220：Human IL-2 ELISA; 1 step and 1hr TBS3221: Human IL-4 ELISA; 1 step and 1hr TBS3223: Human IL-6 ELISA; 1 step and 1hr TBS3224: Human IL-7 ELISA; 1 step and 1hr TBS3225: Human IL-8 ELISA; 1 step and 1hr TBS3226: Human IL-10 ELISA; 1 step and 1hr TBS3227: Human IL-13 ELISA; 1 step and 1hr TBS3228: Human IL-17A ELISA; 1 step and 1hr TBS3229: Human IL-22 ELISA;...

24 Sep
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