HT Medium

HT Medium

The HT Medium is a specialized hybridoma growth medium designed to support hybridoma expansion and monoclonal antibody production in research applications. This chemically defined, animal component-free formulation ensures consistency and reduces the risk of variability or contamination, making it ideal for reliable performance in laboratory-based workflows. Its optimized composition supports efficient cell proliferation and antibody yield following hybridoma selection.

Formulated with hypoxanthine and thymidine (HT), this medium is specifically optimized for weaning hybridomas off aminopterin following HAT selection. It provides a supportive environment for robust hybridoma expansion and downstream antibody production. Validated for use with mouse and rat hybridomas, the medium includes essential components such as L-glutamine, gentamicin, and phenol red, ensuring cell health and growth consistency. The absence of serum or animal-derived components enhances reproducibility and aligns with current expectations for chemically defined media.

Available in a 500 mL liquid format, the HT Medium should be stored at -20°C and can be kept at 2–8°C for up to two weeks after thawing. It is intended strictly for research use only and is not suitable for clinical or therapeutic applications. With clear preparation guidelines and consistent batch performance, this medium serves as a dependable solution for hybridoma researchers aiming to produce monoclonal antibodies for experimental.