Tribo™ Human IFN γ ELISA KitTribioscience
Interferon-gamma (IFN-γ, also known as type II interferon) is an important immunoregulatory cytokine through its antiviral activity. IFN- γ is produced by a number of cell types under inflammatory conditions, including dendritic epidermal cells, T cells, keratinocytes, peripheral blood T cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, NK cells, CD4+T cells, and testicular spermatids. It plays a key role in host defense, and the progression of inflammatory diseases such as autoimmunity and atherosclerosis by exerting anti-viral, anti-proliferative, and immunoregulatory activities.
The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It was designated to make the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, instead of 5-6 hours (Fig. 1). The detection arrangement is from 20 to 15000 pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.