Pfu DNA Polymerase
$159.00
Pfu DNA polymerase is purified from E. coli carrying a vector with the gene encoding Pyrococcus furiosus DNA polymerase. It has intrinsic 3′ to 5′ exonuclease activity and is a highly accurate form of DNA polymerase when compared with Taq DNA polymerase (about 10 fold).
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 503-050 | Yes | 500 U | $159.00 |
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Description
Pfu DNA polymerase is purified from E. coli carrying a vector with the gene encoding Pyrococcus furiosus DNA polymerase. It has an intrinsic 3′ to 5′ exonuclease activity. It is a highly accurate DNA polymerase than Taq DNA polymerase (about 10-fold).
Applications
- High-fidelity PCR and primer-extension reactions.
- PCR products for cloning and expression.
- PCR cloning and blunt-end amplification.
- Gene synthesis.
- Site-directed mutagenesis.
Key Features
- High fidelity PCR: Proofreading activity with 3′ → 5′ exonuclease.
- High specificity: Better specificity than Taq DNA polymerase with reduced chance of non-specific products.
- Terminal transferase activity: Guaranteed blunt-ended PCR product by blocking terminal transferase activity.
Additional information
| SIZE | 500 U |
|---|
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